How is Inotuzumab structurally different from other anti-CD22 antibodies?

Inotuzumab is a humanized antibody targeting CD22. It consists of murine variable regions attached to human constant regions, enhancing compatibility with the human immune system to minimize immunogenicity.

Can Inotuzumab be used for flow cytometry applications?

Yes, Inotuzumab can be used for flow cytometry to detect CD22 expression on B cells. It is recommended to validate its performance under specific experimental conditions.

How does Inotuzumab interact with CD22 on the cellular level?

Inotuzumab specifically binds to the extracellular domain of CD22, a B cell-specific sialoglycoprotein, leading to internalization and potential modulation of cellular functions.

Humanized Anti-CD22 Recombinant Antibody (clone Inotuzumab)

CAT#: TAB-198

This product is a humanized (from mouse) IgG4, κ antibody that can recognize human CD22.

Tested Data Gene Expression

ELISA

Figure 1 Anti-CD22 Antibody (TAB-198) in ELISA

ELISA analysis of TAB-198 was performed by coating with CD22 Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)

Figure 2 Anti-CD22 Antibody (TAB-198) in WB

Western blot analysis of TAB-198 was performed with CD22 Protein, Human, Recombinant (His Tag).
TAB-198 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.6μg)

Figure 3 Anti-CD22 Antibody (TAB-198) in DB

Dot blot analysis of TAB-198 was performed with CD22 Protein, Human, Recombinant (His Tag).
TAB-198 incubation concentration: 2ng/μL.
NC: Negative control PC: Positive control

Figure 4 Anti-CD22 Antibody (TAB-198) in Flow Cytometry

Flow cytometry analysis of Daudi cells with anti-CD22 antibody (Cat#TAB-198, Blue line) or isotype control antibody (Yellow line). A Goat Anti-human IgG (FITC) was used as the secondary antibody.

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Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg

Normal Tissue Figure 2 Spleen

Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22

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Specifications

Immunogen
Human tonsil lymphocytes

Host Species
Human

Derivation
Humanized (from mouse)

Type
Humanized (from mouse) IgG4, κ

Specificity
Human CD22

Species Reactivity
Human

Clone
Inotuzumab

Applications
ELISA, FC, IP, FuncS, IF, Neut, ICC

Product Property

Purification
Protein A/G Purified

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunoprecipitation, Functional Assay, Immunofluorescence, Neutralization, Immunocytochemistry.

Target

Alternative Names
SIGLEC2; SIGLEC-2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; sialic acid-binding Ig-like lectin 2

Gene ID
933

UniProt ID
P20273

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Breast cancer biomarkers at key points during disease progression

Good for Immunofluorescence

We conjugated Inotuzumab with PE and used it in immunofluorescence. It provided clear and specific staining of B cells. Highly recommend.

Scalability for Large Studies

Able to scale up usage for large cohort studies without compromising on performance. Supports extensive research applications.

Minimal Cross-reactivity

We tested Inotuzumab for cross-reactivity, and it showed minimal non-specific binding. Great for our human-specific studies.

Q&As

How is Inotuzumab structurally different from other anti-CD22 antibodies?

A: Inotuzumab is a humanized antibody targeting CD22. It consists of murine variable regions attached to human constant regions, enhancing compatibility with the human immune system to minimize immunogenicity.
Can Inotuzumab be used for flow cytometry applications?

A: Yes, Inotuzumab can be used for flow cytometry to detect CD22 expression on B cells. It is recommended to validate its performance under specific experimental conditions.
How does Inotuzumab interact with CD22 on the cellular level?

A: Inotuzumab specifically binds to the extracellular domain of CD22, a B cell-specific sialoglycoprotein, leading to internalization and potential modulation of cellular functions.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-198, RRID: AB_3111867)

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Biosimilar Overview

Please refer to Inotuzumab Ozogamicin Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Inotuzumab Ozogamicin.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-198.pdf

MSDS

MSDS - TAB-198.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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